Railway companies of Pennsylvania

List of railway companies in the American state of Pennsylvania.

Companies based in the United States

 * Amtrak (AMTK), passenger on freight lines
 * Chesapeake Southern Railway (CHS), freight
 * Conrail (CR), freight and passenger
 * New York Western Railroad (NYW), freight
 * Pennsylvania Seaboard Railroad (PS), freight

Companies based outside of the United States

 * Canadian National Railway (CN), freight

Regional railways

 * Buffalo & Pittsburgh Railroad (BPRR), freight
 * New York, Susquehanna & Western Railway (NYSW), freight
 * Wheeling & Lake Erie Railway (WLE), freight

Shortline and terminal railways

 * Allegheny Valley Railroad (AVR), freight
 * Aliquippa & Ohio River Railroad (AOR), freight
 * Allentown & Auburn Railroad (ALLN), freight
 * Belvidere & Delaware River Railway (BDRV), freight
 * Brandywine Valley Railroad (BVRY), freight
 * Chestnut Ridge Railroad (CHR), freight
 * Columbia & Reading Railway (CORY), freight
 * Conshohocken Recycling and Rail Transfer (CRRT), freight
 * Cumberland Mine Railroad (CMCR), freight
 * Delaware-Lackawanna Railroad (DL), freight
 * East Berlin Railway (EBRY), freight
 * East Broad Top Railroad (EBT), freight and passenger; 3' gauge
 * East Erie Commercial Railroad (EEC), freight
 * East Penn Railroad (ESPN), freight
 * Eastern States Railroad (EASR), freight
 * Elizabethtown Industrial Railroad (EZR), freight
 * Everett Railroad (EV), freight
 * Gettysburg & Northern Railroad (GET), freight
 * Ithaca Central Railroad (ITHR), freight
 * Juniata Terminal Company (JTFS), freight
 * Juniata Valley Railroad (JVRR), freight
 * Kasgro Rail Lines (KRL), freight
 * Kiski Junction Railroad (KJR), freight
 * Landisville Railroad (LVR), freight
 * Lehigh Railway (LRWY), freight
 * Leetsdale Industrial Terminal Railway (LIT), freight
 * Luzerne & Susquehanna Railway (LS), freight
 * Lycoming Valley Railroad (LVRR), freight
 * Maryland Midland Railway (MMID), freight
 * Middletown & Hummelstown Railroad (MIDH), freight and passenger
 * New Castle Industrial Railroad (NCIR), freight
 * New Hope & Ivyland Railroad (NHIR), freight and passenger
 * NJ Transit Rail Operations (NJTR), passenger
 * SEPTA Regional Rail (SEPA), passenger
 * Nittany & Bald Eagle Railroad (NBER), freight
 * NDC Railroad (NDC), freight
 * North Shore Railroad (NSHR), freight
 * Oil Creek & Titusville Railroad (OCTL), freight and passenger
 * Pennsylvania & Southern Railway (PSCC), freight
 * Pennsylvania Northeastern Railroad (PNE), freight
 * Pennsylvania Southwestern Railroad (PSWR), freight
 * Philadelphia Belt Line Railroad (PBL), freight and passenger
 * Pittsburgh, Allegheny & McKees Rocks Railroad (PAM), freight
 * Pittsburgh & Ohio Central Railroad (POHC), freight
 * Reading, Blue Mountain & Northern Railroad (RBMN), freight and passenger
 * Shamokin Valley Railroad (SVRR), freight
 * SMS Rail Lines (SLRS), freight
 * Southwest Pennsylvania Railroad (SWP), freight
 * Strasburg Rail Road (SRC), freight and passenger
 * Tyburn Railroad (TYBR), freight
 * Union Railroad (URR), freight
 * Union County Industrial Railroad (UCIR), freight
 * Upper Merion & Plymouth Railroad (UMP), freight
 * Wellsboro & Corning Railroad (WCOR), freight
 * Western New York & Pennsylvania Railroad (WNYP), freight
 * York Railway (YRC), freight
 * Youngstown & Southeastern Railroad (YSRR), freight

Defunct railways
Years shown here refer to the period in which a given company operated in Pennsylvania; they may have existed elsewhere before or after these years.