Railway companies of West Virginia

List of railway companies in the American state of West Virginia.

Class I railways

 * Amtrak (AMTK), passenger on freight lines
 * Chesapeake Southern Railway (CHS), freight
 * Conrail (CR), freight and passenger
 * New York Western Railroad (NYW), freight
 * Pennsylvania Seaboard Railroad (PS), freight
 * Piedmont & Northern Railway (PN), freight and passenger

Regional railways

 * Wheeling & Lake Erie Railway (WLE), freight

Shortline and terminal railways

 * South Branch Valley Railroad (SBVR), freight and passenger

Defunct railways
Years shown here refer to the period in which a given company operated in West Virginia; they may have existed elsewhere before or after these years.