Railway companies of Florida

List of railway companies in the American state of Florida.

Class I railways

 * Amtrak (AMTK), passenger on freight lines
 * Florida East Coast Railway (FEC), freight and passenger
 * Chesapeake Southern Railway (CHS), freight
 * Pennsylvania Seaboard Railroad (PS), freight
 * Piedmont & Northern Railway (PN), freight and passenger
 * Union Pacific Railroad (UP), freight

Regional railways

 * Alabama & Gulf Coast Railway (AGR), freight
 * Atlanta & St. Andrews Bay Railroad (ASAB), freight

Shortline and terminal railways

 * Florida Midland Railroad (FMID), freight

Defunct railways
Years shown here refer to the period in which a given company operated in Florida; they may have existed elsewhere before or after these years.