Railway companies of Florida

List of railway companies in the American state of Florida.

Class I railways

 * Amtrak (AMTK), passenger on freight lines
 * Florida East Coast Railway (FEC), freight and passenger
 * Chesapeake Southern Railway (CHS), freight
 * Pennsylvania Seaboard Railroad (PS), freight
 * Piedmont & Northern Railway (PN), freight and passenger
 * Union Pacific Railroad (UP), freight

Regional railways

 * Alabama & Gulf Coast Railway (AGR), freight
 * Atlanta & St. Andrews Bay Railroad (ASAB), freight
 * Florida Gulf & Atlantic Railroad (FGAR), freight and passenger

Shortline and terminal railways

 * Apalachicola Northern Railroad (AN), freight
 * Brightline Florida (BLF), high-speed passenger
 * First Coast Railroad (FCRD), freight
 * Florida Central Railroad (FCEN), freight and passenger
 * Florida Midland Railroad (FMID), freight
 * Florida Northern Railroad (FNOR), freight
 * Georgia & Florida Railway (GFRR), freight
 * Jacksonville Port Terminal Railroad (JXPT), freight
 * Port of Panama City Railroad (PPCR), freight
 * Seminole Gulf Railway (SGLR), freight and passenger
 * South Central Florida Express (SCXF), freight
 * US Sugar Railroad (USSC), freight

Defunct railways
Years shown here refer to the period in which a given company operated in Florida; they may have existed elsewhere before or after these years.