Railway companies of West Virginia

List of railway companies in the American state of West Virginia.

Class I railways

 * Amtrak (AMTK), passenger on freight lines
 * Chesapeake Southern Railway (CHS), freight
 * Conrail (CR), freight and passenger
 * New York Western Railroad (NYW), freight
 * Pennsylvania Seaboard Railroad (PS), freight
 * Piedmont & Northern Railway (PN), freight and passenger

Regional railways

 * Wheeling & Lake Erie Railway (WLE), freight

Shortline and terminal railways

 * Appalachian & Ohio Railroad (AO), freight
 * Beech Mountain Railroad (BEEM), freight
 * Durbin & Greenbrier Valley Railroad (DGVR), freight
 * Elk River Railroad (ELKR), freight
 * Kanawha River Railroad (KNWA), freight
 * Kanawha River Terminal Railroad (KRT), freight
 * Little Kanawha River Rail (LKRR), freight
 * MARC Train (MARC), passenger on freight lines
 * South Branch Valley Railroad (SBVR), freight and passenger
 * West Virginia Central Railroad (WVC), freight
 * Winchester & Western Railroad (WW), freight

Defunct railways
Years shown here refer to the period in which a given company operated in West Virginia; they may have existed elsewhere before or after these years.