Railway companies of Ohio

List of railway companies in the American state of Ohio.

Companies based in the United States

 * Amtrak (AMTK), passenger on freight lines
 * Chesapeake Southern Railway (CHS), freight
 * Conrail (CR), freight and passenger
 * New York Western Railroad (NYW), freight
 * Pennsylvania Seaboard Railroad (PS), freight
 * Pere Marquette Railway (PM), freight

Companies based outside of the United States

 * Canadian National Railway (CN), freight

Regional railways

 * Indiana & Ohio Railway (IORY), freight
 * Wheeling & Lake Erie Railway (WLE), freight

Shortline and terminal railways

 * Akron Barberton Cluster Railway (AB), freight
 * Ashland Railway (ASRY), freight
 * Ashtabula, Carson & Jefferson Railroad (ACJR), freight
 * Camp Chase Industrial Railroad (CAMY), freight
 * Central Railroad of Indiana (CIND), freight
 * Chicago, Fort Wayne & Eastern Railroad (CFE), freight
 * Cincinnati East Terminal Railway (CET), freight
 * Cleveland Commercial Railroad (CCR), freight
 * Cleveland Harbor Belt Railroad (CHB), freight
 * Cleveland Works Railway (CWRO), freight
 * Columbus & Ohio River Railroad (CUOH), freight
 * Eastern States Railroad (EASR), freight
 * Flats Industrial Railroad (FIR), freight
 * Grand River Railway (GRRY), freight
 * Indiana Eastern Railroad (IERR), freight
 * Indiana Northeastern Railroad (IN), freight
 * Kanawha River Railroad (KNWA), freight
 * Lake Terminal Railroad (LT), freight
 * Lebanon Mason Monroe Railroad (LMM), freight and passenger
 * Lorain Northern Railroad (LNOR), freight
 * Mahoning Valley Railway (MVRY), freight
 * Napoleon, Defiance & Western Railroad (NDW), freight
 * Newburg & South Shore Railroad (NSR), freight
 * Northern Ohio & Western Railway (NOW), freight
 * Ohio Central Railroad (OHCR), freight
 * Ohio South Central Railroad (OSCR), freight
 * Ohio Southern Railroad (OSRR), freight
 * Ohio Terminal Railway (OHIO), freight
 * Toledo, Lake Erie & Western Railway (TLEW), freight and passenger
 * Republic N&T Railroad (NTRY), freight
 * Warren & Trumbull Railroad (WTRM), freight
 * Youngstown & Austintown Railroad (YARR), freight
 * Youngstown Belt Railroad (YB), freight
 * Youngstown & Southeastern Railroad (YSRR), freight

Defunct railways
Years shown here refer to the period in which a given company operated in Ohio; they may have existed elsewhere before or after these years.