Railway companies of the District of Columbia

List of railway companies in the American District of Columbia.

Class I railways

 * Amtrak (AMTK), passenger on freight lines
 * Chesapeake Southern Railway (CHS), freight
 * Pennsylvania Seaboard Railroad (PS), freight

Shortline and terminal railways

 * MARC Train (MARC), passenger on freight lines
 * Virginia Railway Express (VRE), passenger on freight lines

Defunct railways
Years shown here refer to the period in which a given company operated in the District of Columbia; they may have existed elsewhere before or after these years.